Journal: Nature biomedical engineering

Article Title: A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma.

doi: 10.1038/s41551-022-00919-w

Figure Lengend Snippet: Fig. 1 | Overview of multiplexed electrochemical sensor system. a, Overview of the microfluidic chip designed for an LOC sample-to-answer saliva detection of SARS-CoV-2 RNA and antibodies. (1) The user inputs saliva onto the antibody detection reservoir and a saliva and proteinase K mixture into the sample preparation reservoir, where it incubates. (2) The saliva is pumped over the PES membrane inside the reaction chamber for RNA capture and heated to denature potential reaction inhibitors. (3) The LAMP solution is then pumped from the reservoir into the reaction chamber and incubated. (4) The CRISPR mixture is pumped into the reaction chamber, incubated and then pumped over the EC sensor chip. (5) The saliva for antibody detection is pumped over the EC sensor chip. (6) After the addition of polystreptavidin-HRP and TMB, results from the EC sensor chip are read with a potentiostat. b, An exploded view of the multiplexed system, which includes a heater system, a sealed microfluidic chip and a multiplexed EC sensor chip. c, Photograph of the microfluidic system with a quarter dollar for scale.

Article Snippet: A microfluidic chip for a point-of-care diagnostic that integrated the multiplexed chip was designed using Autocad software and printed on a Formlabs Form 3B 3D SLA printer in grey resin (Version 4; Formlabs RS-F2-GPGR-04).

Techniques: Sample Prep, Membrane, Incubation, CRISPR

Journal: PLoS ONE

Article Title: A Microfluidic Platform for Correlative Live-Cell and Super-Resolution Microscopy

doi: 10.1371/journal.pone.0115512

Figure Lengend Snippet: Left, top: The cell culture is performed under sterile conditions. The flow is established using gravity-driven flow and the flow rate is adjusted by changing the value of ΔH. Left bottom: Cells are introduced into the microfluidic chip (seeding), allowed to attach (attachment), and then maintained under steady, continuous perfusion (growth). Right, top: During the data acquisition phase, the chip is removed from the sterile environment, placed on the microscope stage, and connected to a computer-controlled fluid delivery system. The number of reagents can be adjusted by adding or removing valves. Pressurized air is coupled to reagents which are routed through the system using solenoid pinch valves. Right, bottom: Each reagent can be delivered to each channel in the microfluidic chip through a binary multiplexer tree. By adjusting the configuration of the valves, fluid can be routed to different channels (shown are two examples). A total of n-1 three-way solenoid valves are needed to split the incoming fluid from one channel to n channels.

Article Snippet: Microfluidic chips with miniaturized imaging chambers were designed using AutoCAD (Autodesk, Inc., Sausalito, CA) and fabricated by the Stanford Microfluidics Foundry in PDMS (RTV615, General Electric) using soft lithography.

Techniques: Cell Culture, Sterility, Microscopy

Journal: bioRxiv

Article Title: Droplet microfluidic PicoSorter for high throughput and active selection of cellulolytic microorganisms

doi: 10.1101/2025.09.20.677519

Figure Lengend Snippet: The design of the PicoSorter double-layered chip for picoinjection combined with absorbance-activated droplet sorting – AADS (A) . The PicoSorter module includes: 1. Reinjection droplet chamber, 2. Spacing oil channel, 3. Moat channel with ground electrodes for picoinjection, 4. Positive electrode for picoinjection, 5. Picoinjection buffer. 6. RI-matching oil channel, 7. Optical fiber channels, 8. Bias oil channel, 9 Ground electrode for sorting, 10. Positive electrode for sorting, 11. Positive outlet, 12. Negative outlet. Various important features of the PicoSorter device are indicated in the close-up figure. The first layer (light blue) comprises the droplet reinjection and the channel with a height of 40 µm, from where the droplets are brought to the picoinjection section. The second layer (dark blue), with a height of 90 µm, is where the transmittance detection of droplets was executed. Snapshot of the picoinjection and sorting process (B) . The microphotograph depicts the consecutive microfluidic steps performed with the PicoSorter: 13. Reinjection of 50 pl droplets, 14. Picoinjection of the buffer required for the assay and mixing, 15. Transmittance detection, 16. Active sorting of 100 pl droplets.

Article Snippet: The microfluidic devices used in this study were designed in AutoCAD (Autodesk), and master molds were generated through standard photolithography.

Techniques:

Journal: bioRxiv

Article Title: Droplet microfluidic PicoSorter for high throughput and active selection of cellulolytic microorganisms

doi: 10.1101/2025.09.20.677519

Figure Lengend Snippet: The scatter plot ( A ) illustrates the relationship between the medium pH and buffer (MOPS/NaCl, pH 7) and the transmittance measurement of CMC content at 530 nm. On-chip detection of 100 pL droplets demonstrated that the microfluidic assay can be effectively performed within the suggested pH range. The scatter plot ( B ) depicts the relationship between CMC concentration and the corresponding absorbance-derived voltage signal at 530 nm, measured at pH 7. An inverse relationship is observed, with increasing CMC concentration resulting in a decrease in the detected voltage signal. The influence of picoinjection of buffer solution on droplet transmittance values is shown in the histogram on the right ( C ), where the absorbance signal of each droplet was quantified and the droplets enumerated. The graphs show results for different droplet populations containing cellulolytic cultures of C. cellulans (λ∼0.1). The green histogram represents transmittance measurements of 50 pL droplets that were merged with 50 pL of MOPS/NaCl buffer, while the light red histogram corresponds to 100 pL droplets measured without buffer injection.

Article Snippet: The microfluidic devices used in this study were designed in AutoCAD (Autodesk), and master molds were generated through standard photolithography.

Techniques: Concentration Assay, Derivative Assay, Injection

Journal: bioRxiv

Article Title: Droplet microfluidic PicoSorter for high throughput and active selection of cellulolytic microorganisms

doi: 10.1101/2025.09.20.677519

Figure Lengend Snippet: The C. cellulans emulsion (λ ∼ 0.1) was incubated at 25°C under dynamic droplet conditions and therefore screened. Microfluidic operations simultaneously performed in the PicoSorter device are shown on the left ( A ). A clonal colony of C. cellulans growing inside a 50 pL droplet first undergoes picoinjection of buffer, after which the resulting 100 pL droplet is sorted at a throughput of 0.6 kHz ( B ).

Article Snippet: The microfluidic devices used in this study were designed in AutoCAD (Autodesk), and master molds were generated through standard photolithography.

Techniques: Emulsion, Incubation

Journal: bioRxiv

Article Title: Rapid and reusable high-throughput microfluidics through modular assembly

doi: 10.64898/2026.01.12.699088

Figure Lengend Snippet: Design and workflow of the modular microfluidic platform. A. Schematic of the device and platform assembly illustrating the multilayer components. The complete assembly is designed to fit within an atmospheric control box for cell culture. B . Picture of a fully assembled device loaded with food dye. Red indicates control channels, while blue indicates fluid channels and cell chambers. C. Flowchart comparing the proposed modular assembly method (red arrows) with the conventional approach (black arrows). For multilayer chip fabrication, mandatory steps include (i) mixing/degassing PDMS for control layer, (ii) PDMS spin coating for fluid layer, (iii) overnight baking, (iv) surface plasma treatment; (v) aligning and bonding; (vi) hole punching. Our modular design mitigates assembly-related failures and enables rapid reuse of the chip within 2 h, thereby substantially increasing experimental success rates and effective throughput.

Article Snippet: All microfluidic device components were designed using Autodesk 2025, Onshape, AutoCAD (Autodesk Inc.), or KLayout.

Techniques: Control, Cell Culture, Clinical Proteomics

Journal: bioRxiv

Article Title: Rapid and reusable high-throughput microfluidics through modular assembly

doi: 10.64898/2026.01.12.699088

Figure Lengend Snippet: Fabrication and assembly of the modular microfluidic platform. A. Schematic workflow for fabricating the reusable PDMS fluid-control module. Control holes are punched prior to alignment. B. Workflow for the thin-substrate. A hybrid adhesive polymer (mixture of adhesive silicone and PDMS) is spin-coated onto a glass slide and cured to create a reversible sealing interface. C. Workflow for the deep-well substrate for 3D culture. Using a 3D-printed master, 1 mm deep chambers are molded in PDMS with a glass slide on top. D. Cross-sectional diagrams of the final device assembly. The fluid-control module and deep-well substrate are manually aligned using outlines incorporated into the design and the corresponding inset in the aluminum tray, then mechanically clamped. Negative pressure can be applied to further increase flow rate.

Article Snippet: All microfluidic device components were designed using Autodesk 2025, Onshape, AutoCAD (Autodesk Inc.), or KLayout.

Techniques: Control, Adhesive, Polymer